- In consultation with the Principal Investigator, designs and conducts bench-level experiments and lead small independent research projects.
- Execute laboratory experiments, which may include techniques such as:o
- Performs analysis of experimental results by identifying methodological problems and helping to identify and implement modifications in research protocols.
- Documents, compiles, and assists in interpreting experimental data and participates in lab meetings.
- Maintains accurate, up-to-date records of cell lines, primary tissue samples, and experimental samples.
- Independently operate, manage, and maintain complex laboratory equipment, including liquid-handlers, Illumina sequencers, and smaller instruments
- Participates in general laboratory responsibilities, including ordering and organizing laboratory supplies.
- Other tasks as required or assigned.
- Ideally, 2 years of experience working in a research laboratory.
- For Master's degree, at least one year of directly related, full-time lab experience.
- Proficient in basic lab techniques required.
- Must be reliable, organized, and able to work independently as well as with other members of the team and research center
- Excellent written and oral communication skills, as well as strong organizational skills required.
- Proficiency with computer software, including web-based applications and some Microsoft Office applications (Word, Excel, PowerPoint) required.
- Knowledge of various scientific databases and publications
- Previous project management experience
- Familiarity with bioinformatics tools (Python, R, or equivalent), and/or programming language (C/C++, Java)
- Ideally, 2 years of experience working in a research laboratory.
- For Master's degree, at least one year of directly related, full-time lab experience.
- Proficient in basic lab techniques required.
- Must be reliable, organized, and able to work independently as well as with other members of the team and research center
- Excellent written and oral communication skills, as well as strong organizational skills required.
- Proficiency with computer software, including web-based applications and some Microsoft Office applications (Word, Excel, PowerPoint) required.
- Knowledge of various scientific databases and publications
- Previous project management experience
- Familiarity with bioinformatics tools (Python, R, or equivalent), and/or programming language (C/C++, Java)
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Research Technician II - Auckland, New Zealand - Massachusetts General Physicians' Organization
Description
Job Description - Research Technician IIResearch Technician II
-(
3280441
)
A laboratory research technician position is available in Dr.
Alexandra-Chloé Villani research at the MGH Center for Immunology and Inflammatory Diseases (CIID), where she also directs the Single Cell Genomics Research Program.
Dr.Villani leads a multidisciplinary research program focuses on developing and implementing systems immunology and genomics strategies to further our understanding of the rules of human immune tolerance in maintaining health and promoting immune-related diseases.
The Villani laboratory is also affiliated to the Broad Institute of MIT & Harvard, as well as the MGH Krantz Family Center for Cancer Research, offering additional opportunities to collaborate and bridge with this vibrant community.
This position offers the opportunity to learn and employ the cutting-edge techniques in genomics, systems immunology, and single cell genomics to solve important problems in health and disease related to the human immune system, autoimmune diseases and cancer.
Single cell genomics is revolutionizing our understanding of biology – from redefining our understanding of the types of cells, a fundamental unit in biology, to translating this knowledge to better understand disease phenotypes and the implications of this to therapeutics.
We are seeking a bright, enthusiastic individual, with a strong interest in immunology and molecular biologyto join our efforts. This position represents an exciting opportunity to work as a member of
an interdisciplinary team of
biologists, laboratory scientists,
computational biologists,
and physicians working together on transformative translational efforts that are bridging between the clinical and research interfaces.
Our research program is developing and implementing unbiased experimental and computational strategies that can directly survey the human immune system in order to define at high resolution the key processes and players underlying healthy human immune responses as a foundation for understanding how immunity is dysregulated in diseases.
This includes establishing a more comprehensive roadmap of the human immune system through identifying novel immune cell subpopulations across tissues using single-cell 'multi-omics' strategies along with mapping the cellular ecosystem and associated molecular circuitry driving immune diseases.
Collectively,our research program is
empowering the study of the human immune system as a function of "healthy" baseline, inflammatory state, disease progression, and response to treatment with emphasis on precision medicine, ultimately paving the way for developing a comprehensive human immune lexicon that is key to promoting effective bench-to-beside translation of findings.
the research associate hired will be performing a variety of techniques in both BL1, BL2 and BL2+ environments.
These will include but are not limited to human and mice primary tissue processing, flow cytometry and cell sorting, single-cell RNA/DNA/protein/ATAC-sequencing data generation, spatial transcriptomics experiments, sequencing library construction, bulk RNA extraction and analysis, by RT-PCR or sequencing, cell cultures for in vitro and in vivo perturbation experiments, CRISPR screens, western blotting, immunohistochemistry and image analysis.
The incumbent is expected to work both independently and as a team member with investigators, staff, clinicians, fellows, and students.
The Massachusetts General Hospital Single Cell Genomics Research Program is a new multi-disciplinary translational research group with the MGH Center for Immunology and Inflammatory Diseases focused on developing and implementing systems immunology and cutting-edge genomics strategies to further our understanding of the human immune system in health and disease.
We are leveraging these strategies to redefine "precision medicine" approaches, including using results from the laboratory to help inform the development of the next-generation of clinical trials.
The Massachusetts General Hospital (MGH), a leading medical center located in the heart of Boston, is consistently ranked among the top hospitals in America.
MGH is the original and largest teaching hospital of Harvard Medical School.We are also affiliated with the MGH Department of Medicine, the MGH Cancer Center, Harvard Medical School, as well as with the Broad Institute.
Working in our group offers the opportunity to learn and employ the cutting-edge techniques in genomics, single cell genomics, human immunology, and systems immunology to solve important problems in health and disease related to the human immune system, autoimmune diseases, and cancer.
This position also represents an exciting opportunity to work as a member of an interdisciplinary team of biologists, laboratory scientists, computational biologists, and physicians working together on transformative translational efforts that are bridging between the clinical and research interfaces.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
o
Single-cell
RNA/DNA/protein/ATAC-sequencing
data generation techniques through plate-based and droplet-based approaches (not essential to know the techniques prior to applying for this position)
o
Assisting with FACS analyses and cell sorting
o
Processing and maintaining cell cultures
o
In vitro functional experiments of primary human immune cells
o
Molecular biology techniques, including DNA and RNA isolation, PCR, and quantitative PCR, sequencing library construction
o
Immunostaining for flow cytometry and microscopy
New graduates with some lab experience (via course work, internships, etc.) or those without any prior research experience will be considered for the Research Technician I position and are welcomed to apply.
.
Bachelor's degree in Biology, Biochemistry, Immunology, Molecular Biology, Life Science, Engineering, or a related scientific field required.
New graduates with some lab experience (via course work, internships, etc.) or those without any prior research experience will be considered for the Research Technician I position and are welcomed to apply.
.
Massachusetts General Hospital is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead.
All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law.
We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.
Primary LocationPrimary Location
MA-Charlestown-MGH 529 Main
Work Locations
MGH 529 Main
529 Main Street
Charlestown
02129
Job
Organization
Organization
Massachusetts General Hospital(MGH)
Schedule
Schedule
Full-time
Standard Hours
40
Shift
Shift
Day Job
Employee Status
Employee Status
Regular
Recruiting Department
MGH Center for Immunology and Inflammatory Disease
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